top of page
bhaskar napte
Dec 6, 20233 min read
Why You Must Avoid Highly Aqueous Mobile Phases When Using C8/C18 Columns?
This article delves into the challenges due to the usage of high aq. mobile phases in RPLC and offers solutions
2,0051
bhaskar napte
Dec 1, 20232 min read
How to Select the Dimension of an HPLC Column?
Selecting the right HPLC column dimensions during HPLC method development
1,7433
bhaskar napte
Nov 30, 20233 min read
How to reduce mobile phase consumption during an HPLC analysis
How to reduce mobile phase consumption during HPLC analysis
7201
bhaskar napte
Nov 27, 20232 min read
💥Effect of Triethylamine (TEA) on the Retention in RPLC
Impact of Triethylamine on Retention, and Peak Tailing in HPLC analysis
3,2432
bhaskar napte
Nov 23, 20232 min read
What is the effect of free silanols in RPLC and how to reduce it?
Understand the silanol effect and way out to minimize it in RPLC
1,4041
bhaskar napte
Nov 15, 20232 min read
Are the Tailing factor, Symmetry factor, and Asymmetry factor the same?
tailing factor assymetry factor and symmtery factor as per USP and EP or Ph. Eur
4,5870
bhaskar napte
Nov 10, 20233 min read
Why resolution and plate number is measured by using peak width at the half height
measurement of plate number and resolution in HPLC analysis
2060
bhaskar napte
Nov 8, 20234 min read
Maximizing HPLC Column Efficiency: A Comprehensive Guide
This blog will help you to maximize the performance of your HPLC column
4040
bhaskar napte
Nov 7, 20233 min read
Peak Tailing: Phenomenon, Symptoms and Corrections
Peak tailing phenomenon, symptoms, and corrections
2,4241
bhaskar napte
Nov 1, 20233 min read
Mastering Peak Compression: The Key to Sharper Chromatography
Hoe gradient elution can help in peak compression
2120
bhaskar napte
Oct 30, 20234 min read
pH, pKa, and Retention
The role of pH and pKa on retention of acid and base in RPLC
3,0122
bhaskar napte
Oct 20, 20231 min read
Amino Columns in Chromatography
Use of amino column in HPLC method development
840
bhaskar napte
Oct 20, 20233 min read
Retention of Acidic, Basic, and Neutral Compounds in Ion Pair Chromatography
Retention of Acidic, Basic, and Neutral Compounds in Ion Pair Chromatography
1230
bhaskar napte
Oct 20, 20232 min read
Exploring the Surprising Increase in Retention Time of Protonated Bases at Low pH in RPLC
Exploring the Surprising Increase in Retention Time of Protonated Bases at Low pH in RPLC
270
bhaskar napte
Oct 20, 20232 min read
Why does Acetonitrile have higher elution strength than methanol?
We will explore, why Acetonitrile has higher elution strength than methanol.
4,3473
bhaskar napte
Oct 20, 20232 min read
Phenyl Column Mystery
The role of Phenyl column in HPLC method development
1421
bhaskar napte
Oct 20, 20232 min read
Unraveling the Advantages of Core-Shell Columns in HPLC
what core-shell particles are, how they differ from traditional porous particles, their history, and the advantages they bring
760
bhaskar napte
Oct 20, 20232 min read
Methanol vs. Acetonitrile: Which Solvent is Better for Chromatography?
we'll explore the unique properties of methanol and acetonitrile and discuss when and why you might prefer one over the other for HPLC
5470
bhaskar napte
Oct 20, 20232 min read
QbD vs AQbD
Understanding the key terms associated with QbD (Quality by Design) and AQbD (Analytical Quality by Design)
1,9412
bhaskar napte
Oct 20, 20232 min read
Efficient Tips for Column Cleaning in Reverse Phase Liquid Chromatography
highly effective tips for column cleaning, particularly geared toward reverse-phase liquid chromatography
1510
bottom of page